cryo-EM & cryo-ET

CEM
Cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) allow insights into the 2D and 3D structure of biological samples at near atomic resolution. Contrary to conventional EM, samples are rapidly frozen without using chemical fixation or heavy metal staining to visualise samples ranging from proteins to cells close to their native state.

Ressources

  • FEI Tecnai F30 Helium Polara (300 kV transmission electron microscope)
  • Gatan K2 summit direct electron detector
  • Leica EM GP plunge freezer
  • FEI Vitrobot Mk III plunge freezer
  • Leica cryo-CLEM

Operators


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Applications

  • 2D (cryo-EM) and 3D (cryo-ET) visualisation
  • near atomic resolution of biological samples close to their native state
  • suitable for proteins, vesicles, organelles, viruses and cells
  • correlative light and electron microscopy (CLEM)