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Mass Spectrometry Imaging

Mass Spectrometry Imaging
Mass Spectrometry Imaging (MSI) allows a completely untargeted approach towards tissue visualization. Multiple analytes (drugs, metabolites, proteins, lipids …) are recorded in parallel on tissue. MSI has high potential to be the first method to accomplish the unbiased identification of unknown components involved in biological interactions and to identify compartmentalized biological changes.

Ressources

  • Cryostat CryoStar NX50 with steel knife and tungsten carbide knife (16 cm each) in addition to standard knifes
  • sample preparation for MS based imaging (sublimation unit, matrix sprayer, picovolume spotter)
  • (Atmospheric Pressure) Matrix assisted laser desorption/ionization, (AP-)MALDI
  • Desorption Electrospray Ionization, DESI
  • Time-of-Flight Mass Spectrometry, ToF-MS for molecular weight determination
  • ToF/ToF for MS/MS for analyte identification
  • Ion Mobility Separation, IMS
  • software for data interpretation (incl. statistics) and visualization
  • software for multimodal image alignment

Available instrumentation:

  • MALDI-ToF/RToF MS w/ MS/MS capability for maximum structural information (CID @20keV)
  • MALDI-ToF/RToF MS w/ MS/MS capability for high lateral resolution (low µm)
  • AP-MALDI-qToF w/ MS/MS and/or IMS capability for gas phase separation and high mass accuracy (2-5 ppm)
  • DESI-qToF w/ MS/MS and/or IMS capability for gas phase separation and high mass accuracy (2-5 ppm)
  • Operators


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    further information ...

    Applications

    • imaging molecular information on polymer material (e.g explants)
    • imaging of polymer degradation products in tissue
    • imaging of small molecule distributions (drugs, lipids, metabolites, ..) in tissue
    • imaging of peptide/protein/glycoconjugate distributions in tissue
    • imaging of peptides/lipids in cells